Protein Extraction:Article Title: Label-Free Quantitative Proteomic Study of The Effect of Dihydrotestosterone (DHT) On Rat Ovarian GCs
Article Snippet: .. 2.1.2 The main materials and reagents are listed in the following table Page 5/28 Materials and Reagents Company DHT (5α-Dihydrotestosterone-D3) Sigma, USA PMSG (pregnant mare serum gonadotropin) Prospec-Tany, USA DMEM/F-12 culture medium BI, USA Fetal bovine serum BI, USA Penicillin streptomycin BI, USA Whole protein extraction kit KeyGEN, China BCA kit KeyGEN, China PBS HyClone, USA Dithiothreitol Sigma, USA Iodoacetamide Sigma, USA Urea Sigma, USA Trypsin Promega, USA Formic acid Sigma, USA Acetonitrile Fisher Chemical, USA 8-12% SDS-PAGE Gel Keygen, China PVDF membrane Millipore, USA Rabbit pAb Mark3 Abclonal, China Rabbit pAb Mre11a Abclonal, China Rabbit pAb Fth1 Abclonal, China Rabbit pAb Nqo1 Abclonal, China Rabbit pAb β-Actin Abclonal, China HRP Goat Anti-Rabbit IgG Abclonal, China Luminous uid Thermo, USA 2.1.3 Software used for analysis Page 6/28 Analysis Software/method Version/URL Mass spectrometry data analysis MaxQuant v.1.5.2.8 http://www.maxquant.org/ GO comment InterProScan v.5.14-53.0 http://www.ebi.ac.uk/interpro/ KEGG comment KAAS KEGG Mapper v.2.0 http://www.genome.jp/kaas-bin/kaas_main V2.5 http://www.kegg.jp/kegg/mapper.html Enrichment analysis Perl module v.1.31 https://metacpan.org/pod/Text::NSP::Measures::2D::Fisher Subcellular localization Wolfpsort v.0.2 http://www.genscript.com/psot/wolfpsort.html 3 Experimental Method 3.1 Primary rat granulosa cell culture and DHT intervention The 21-day-old SD female rats were injected intraperitoneally with PMSG (10 IU/), and the animals were killed by cervical vertebra dislocation 36 hours later. ..
SDS Page:Article Title: Label-Free Quantitative Proteomic Study of The Effect of Dihydrotestosterone (DHT) On Rat Ovarian GCs
Article Snippet: .. 2.1.2 The main materials and reagents are listed in the following table Page 5/28 Materials and Reagents Company DHT (5α-Dihydrotestosterone-D3) Sigma, USA PMSG (pregnant mare serum gonadotropin) Prospec-Tany, USA DMEM/F-12 culture medium BI, USA Fetal bovine serum BI, USA Penicillin streptomycin BI, USA Whole protein extraction kit KeyGEN, China BCA kit KeyGEN, China PBS HyClone, USA Dithiothreitol Sigma, USA Iodoacetamide Sigma, USA Urea Sigma, USA Trypsin Promega, USA Formic acid Sigma, USA Acetonitrile Fisher Chemical, USA 8-12% SDS-PAGE Gel Keygen, China PVDF membrane Millipore, USA Rabbit pAb Mark3 Abclonal, China Rabbit pAb Mre11a Abclonal, China Rabbit pAb Fth1 Abclonal, China Rabbit pAb Nqo1 Abclonal, China Rabbit pAb β-Actin Abclonal, China HRP Goat Anti-Rabbit IgG Abclonal, China Luminous uid Thermo, USA 2.1.3 Software used for analysis Page 6/28 Analysis Software/method Version/URL Mass spectrometry data analysis MaxQuant v.1.5.2.8 http://www.maxquant.org/ GO comment InterProScan v.5.14-53.0 http://www.ebi.ac.uk/interpro/ KEGG comment KAAS KEGG Mapper v.2.0 http://www.genome.jp/kaas-bin/kaas_main V2.5 http://www.kegg.jp/kegg/mapper.html Enrichment analysis Perl module v.1.31 https://metacpan.org/pod/Text::NSP::Measures::2D::Fisher Subcellular localization Wolfpsort v.0.2 http://www.genscript.com/psot/wolfpsort.html 3 Experimental Method 3.1 Primary rat granulosa cell culture and DHT intervention The 21-day-old SD female rats were injected intraperitoneally with PMSG (10 IU/), and the animals were killed by cervical vertebra dislocation 36 hours later. ..
Membrane:Article Title: Label-Free Quantitative Proteomic Study of The Effect of Dihydrotestosterone (DHT) On Rat Ovarian GCs
Article Snippet: .. 2.1.2 The main materials and reagents are listed in the following table Page 5/28 Materials and Reagents Company DHT (5α-Dihydrotestosterone-D3) Sigma, USA PMSG (pregnant mare serum gonadotropin) Prospec-Tany, USA DMEM/F-12 culture medium BI, USA Fetal bovine serum BI, USA Penicillin streptomycin BI, USA Whole protein extraction kit KeyGEN, China BCA kit KeyGEN, China PBS HyClone, USA Dithiothreitol Sigma, USA Iodoacetamide Sigma, USA Urea Sigma, USA Trypsin Promega, USA Formic acid Sigma, USA Acetonitrile Fisher Chemical, USA 8-12% SDS-PAGE Gel Keygen, China PVDF membrane Millipore, USA Rabbit pAb Mark3 Abclonal, China Rabbit pAb Mre11a Abclonal, China Rabbit pAb Fth1 Abclonal, China Rabbit pAb Nqo1 Abclonal, China Rabbit pAb β-Actin Abclonal, China HRP Goat Anti-Rabbit IgG Abclonal, China Luminous uid Thermo, USA 2.1.3 Software used for analysis Page 6/28 Analysis Software/method Version/URL Mass spectrometry data analysis MaxQuant v.1.5.2.8 http://www.maxquant.org/ GO comment InterProScan v.5.14-53.0 http://www.ebi.ac.uk/interpro/ KEGG comment KAAS KEGG Mapper v.2.0 http://www.genome.jp/kaas-bin/kaas_main V2.5 http://www.kegg.jp/kegg/mapper.html Enrichment analysis Perl module v.1.31 https://metacpan.org/pod/Text::NSP::Measures::2D::Fisher Subcellular localization Wolfpsort v.0.2 http://www.genscript.com/psot/wolfpsort.html 3 Experimental Method 3.1 Primary rat granulosa cell culture and DHT intervention The 21-day-old SD female rats were injected intraperitoneally with PMSG (10 IU/), and the animals were killed by cervical vertebra dislocation 36 hours later. ..
Software:Article Title: Label-Free Quantitative Proteomic Study of The Effect of Dihydrotestosterone (DHT) On Rat Ovarian GCs
Article Snippet: .. 2.1.2 The main materials and reagents are listed in the following table Page 5/28 Materials and Reagents Company DHT (5α-Dihydrotestosterone-D3) Sigma, USA PMSG (pregnant mare serum gonadotropin) Prospec-Tany, USA DMEM/F-12 culture medium BI, USA Fetal bovine serum BI, USA Penicillin streptomycin BI, USA Whole protein extraction kit KeyGEN, China BCA kit KeyGEN, China PBS HyClone, USA Dithiothreitol Sigma, USA Iodoacetamide Sigma, USA Urea Sigma, USA Trypsin Promega, USA Formic acid Sigma, USA Acetonitrile Fisher Chemical, USA 8-12% SDS-PAGE Gel Keygen, China PVDF membrane Millipore, USA Rabbit pAb Mark3 Abclonal, China Rabbit pAb Mre11a Abclonal, China Rabbit pAb Fth1 Abclonal, China Rabbit pAb Nqo1 Abclonal, China Rabbit pAb β-Actin Abclonal, China HRP Goat Anti-Rabbit IgG Abclonal, China Luminous uid Thermo, USA 2.1.3 Software used for analysis Page 6/28 Analysis Software/method Version/URL Mass spectrometry data analysis MaxQuant v.1.5.2.8 http://www.maxquant.org/ GO comment InterProScan v.5.14-53.0 http://www.ebi.ac.uk/interpro/ KEGG comment KAAS KEGG Mapper v.2.0 http://www.genome.jp/kaas-bin/kaas_main V2.5 http://www.kegg.jp/kegg/mapper.html Enrichment analysis Perl module v.1.31 https://metacpan.org/pod/Text::NSP::Measures::2D::Fisher Subcellular localization Wolfpsort v.0.2 http://www.genscript.com/psot/wolfpsort.html 3 Experimental Method 3.1 Primary rat granulosa cell culture and DHT intervention The 21-day-old SD female rats were injected intraperitoneally with PMSG (10 IU/), and the animals were killed by cervical vertebra dislocation 36 hours later. ..
Mass Spectrometry:Article Title: Label-Free Quantitative Proteomic Study of The Effect of Dihydrotestosterone (DHT) On Rat Ovarian GCs
Article Snippet: .. 2.1.2 The main materials and reagents are listed in the following table Page 5/28 Materials and Reagents Company DHT (5α-Dihydrotestosterone-D3) Sigma, USA PMSG (pregnant mare serum gonadotropin) Prospec-Tany, USA DMEM/F-12 culture medium BI, USA Fetal bovine serum BI, USA Penicillin streptomycin BI, USA Whole protein extraction kit KeyGEN, China BCA kit KeyGEN, China PBS HyClone, USA Dithiothreitol Sigma, USA Iodoacetamide Sigma, USA Urea Sigma, USA Trypsin Promega, USA Formic acid Sigma, USA Acetonitrile Fisher Chemical, USA 8-12% SDS-PAGE Gel Keygen, China PVDF membrane Millipore, USA Rabbit pAb Mark3 Abclonal, China Rabbit pAb Mre11a Abclonal, China Rabbit pAb Fth1 Abclonal, China Rabbit pAb Nqo1 Abclonal, China Rabbit pAb β-Actin Abclonal, China HRP Goat Anti-Rabbit IgG Abclonal, China Luminous uid Thermo, USA 2.1.3 Software used for analysis Page 6/28 Analysis Software/method Version/URL Mass spectrometry data analysis MaxQuant v.1.5.2.8 http://www.maxquant.org/ GO comment InterProScan v.5.14-53.0 http://www.ebi.ac.uk/interpro/ KEGG comment KAAS KEGG Mapper v.2.0 http://www.genome.jp/kaas-bin/kaas_main V2.5 http://www.kegg.jp/kegg/mapper.html Enrichment analysis Perl module v.1.31 https://metacpan.org/pod/Text::NSP::Measures::2D::Fisher Subcellular localization Wolfpsort v.0.2 http://www.genscript.com/psot/wolfpsort.html 3 Experimental Method 3.1 Primary rat granulosa cell culture and DHT intervention The 21-day-old SD female rats were injected intraperitoneally with PMSG (10 IU/), and the animals were killed by cervical vertebra dislocation 36 hours later. ..
Cell Culture:Article Title: Label-Free Quantitative Proteomic Study of The Effect of Dihydrotestosterone (DHT) On Rat Ovarian GCs
Article Snippet: .. 2.1.2 The main materials and reagents are listed in the following table Page 5/28 Materials and Reagents Company DHT (5α-Dihydrotestosterone-D3) Sigma, USA PMSG (pregnant mare serum gonadotropin) Prospec-Tany, USA DMEM/F-12 culture medium BI, USA Fetal bovine serum BI, USA Penicillin streptomycin BI, USA Whole protein extraction kit KeyGEN, China BCA kit KeyGEN, China PBS HyClone, USA Dithiothreitol Sigma, USA Iodoacetamide Sigma, USA Urea Sigma, USA Trypsin Promega, USA Formic acid Sigma, USA Acetonitrile Fisher Chemical, USA 8-12% SDS-PAGE Gel Keygen, China PVDF membrane Millipore, USA Rabbit pAb Mark3 Abclonal, China Rabbit pAb Mre11a Abclonal, China Rabbit pAb Fth1 Abclonal, China Rabbit pAb Nqo1 Abclonal, China Rabbit pAb β-Actin Abclonal, China HRP Goat Anti-Rabbit IgG Abclonal, China Luminous uid Thermo, USA 2.1.3 Software used for analysis Page 6/28 Analysis Software/method Version/URL Mass spectrometry data analysis MaxQuant v.1.5.2.8 http://www.maxquant.org/ GO comment InterProScan v.5.14-53.0 http://www.ebi.ac.uk/interpro/ KEGG comment KAAS KEGG Mapper v.2.0 http://www.genome.jp/kaas-bin/kaas_main V2.5 http://www.kegg.jp/kegg/mapper.html Enrichment analysis Perl module v.1.31 https://metacpan.org/pod/Text::NSP::Measures::2D::Fisher Subcellular localization Wolfpsort v.0.2 http://www.genscript.com/psot/wolfpsort.html 3 Experimental Method 3.1 Primary rat granulosa cell culture and DHT intervention The 21-day-old SD female rats were injected intraperitoneally with PMSG (10 IU/), and the animals were killed by cervical vertebra dislocation 36 hours later. ..
Injection:Article Title: Label-Free Quantitative Proteomic Study of The Effect of Dihydrotestosterone (DHT) On Rat Ovarian GCs
Article Snippet: .. 2.1.2 The main materials and reagents are listed in the following table Page 5/28 Materials and Reagents Company DHT (5α-Dihydrotestosterone-D3) Sigma, USA PMSG (pregnant mare serum gonadotropin) Prospec-Tany, USA DMEM/F-12 culture medium BI, USA Fetal bovine serum BI, USA Penicillin streptomycin BI, USA Whole protein extraction kit KeyGEN, China BCA kit KeyGEN, China PBS HyClone, USA Dithiothreitol Sigma, USA Iodoacetamide Sigma, USA Urea Sigma, USA Trypsin Promega, USA Formic acid Sigma, USA Acetonitrile Fisher Chemical, USA 8-12% SDS-PAGE Gel Keygen, China PVDF membrane Millipore, USA Rabbit pAb Mark3 Abclonal, China Rabbit pAb Mre11a Abclonal, China Rabbit pAb Fth1 Abclonal, China Rabbit pAb Nqo1 Abclonal, China Rabbit pAb β-Actin Abclonal, China HRP Goat Anti-Rabbit IgG Abclonal, China Luminous uid Thermo, USA 2.1.3 Software used for analysis Page 6/28 Analysis Software/method Version/URL Mass spectrometry data analysis MaxQuant v.1.5.2.8 http://www.maxquant.org/ GO comment InterProScan v.5.14-53.0 http://www.ebi.ac.uk/interpro/ KEGG comment KAAS KEGG Mapper v.2.0 http://www.genome.jp/kaas-bin/kaas_main V2.5 http://www.kegg.jp/kegg/mapper.html Enrichment analysis Perl module v.1.31 https://metacpan.org/pod/Text::NSP::Measures::2D::Fisher Subcellular localization Wolfpsort v.0.2 http://www.genscript.com/psot/wolfpsort.html 3 Experimental Method 3.1 Primary rat granulosa cell culture and DHT intervention The 21-day-old SD female rats were injected intraperitoneally with PMSG (10 IU/), and the animals were killed by cervical vertebra dislocation 36 hours later. ..
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